Journal: Journal of Diabetes

Article Title: Long noncoding RNAs and metabolic memory associated with continued progression of diabetic retinopathy

doi: 10.1111/1753-0407.70009

Figure Lengend Snippet: Primer sequence.

Article Snippet: The labeled cRNA was hybridized in an Agilent hybridization oven at 65°C for 17 h onto the Rat LncRNA Array v3.0 (4 × 44 K, Arraystar); this allowed assessment of both LncRNA and messenger RNA (mRNA) on the same chip. (Please note: Rat LncRNA Array v3.0 can detect ~10 333 LncRNAs and 28 287 coding transcripts).

Techniques: Sequencing

Journal: Journal of Diabetes

Article Title: Long noncoding RNAs and metabolic memory associated with continued progression of diabetic retinopathy

doi: 10.1111/1753-0407.70009

Figure Lengend Snippet: Long noncoding RNAs (LncRNA)–mRNA coexpression network of selected LncRNA with mRNAs. (A) Coexpression network of differentially expressed LncRNAs. Blue node denotes LncRNA; orange node denotes mRNA coding gene; solid line denotes directed, positive correlation; broken line, undirected, negative correlation. (B) Gene transcripts, quantified by quantitative real‐time polymerase chain reaction (qRT‐PCR) using β‐actin as a housekeeping gene for downregulated and upregulated mRNAs. Values are represented as mean ± SD from 6 to 7 rats/group. Diab, continuous poor glycemic control for 8 months; Norm, normal control; Rev, poor glycemic control for 4 months, followed by good glycemic control for 4 months. * p < 0.05 versus Norm.

Article Snippet: The labeled cRNA was hybridized in an Agilent hybridization oven at 65°C for 17 h onto the Rat LncRNA Array v3.0 (4 × 44 K, Arraystar); this allowed assessment of both LncRNA and messenger RNA (mRNA) on the same chip. (Please note: Rat LncRNA Array v3.0 can detect ~10 333 LncRNAs and 28 287 coding transcripts).

Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Control

Journal: bioRxiv

Article Title: The long non-coding RNA NEAT1 regulates the transcriptional landscape of cardiomyocytes

doi: 10.1101/2024.06.27.600932

Figure Lengend Snippet: (A) Experimental setup to detect the deregulated lncRNA in hearts subjected to HF, HF+AAV9-SERCA2a relative to Sham hearts (B) Microarray based profiling of RNA isolated from rat hearts subjected to Myocardial infarction and rescue by Serca2a gene therapy. (C) qRT-PCR of Neat1 from RNA isolated from rat ventricle after MI and MI+Serca2a gene therapy. (D) qRT-PCR for detecting the change in expression of ANP, β-MHC, Neat1, and Neat1.2 in primary neonatal rat ventricular myocytes (NRCM) challenged with 100 µM Isoproterenol for 48 h. * p value < 0.05, ** p value <0.01. t-tests were performed to compute statistical differences between both groups. (E) Neat1 expression levels detected by qRT-PCR and ejection fraction (EF%) throughout heart failure progression (TAC, transverse aortic constriction; n = 5–8). * p value < 0.05, t-tests were performed to compute statistical differences between Sham and TAC. (F) Neat1 expression levels and ejection fraction (EF%) throughout heart failure progression after subjecting mice to 15 mg/kg/day, 30mg/kg/day, and 50 mg/kg/day of isoproterenol for 10 days. * p value < 0.05, ** p value <0.01. t-tests were performed to compute statistical differences between saline and isoproterenol groups. (G) qRT-PCR for detecting the expression of total Neat1 and Neat1.2 in adult mice cardiomyocytes and adult mice non-myocyte fraction.

Article Snippet: Rat lncRNA microarray (4X44K, Arraystar) was performed in the heart samples isolated from the rats that were subjected to proximal coronary artery ligation (HF), HF+ SERCA2A gene therapy.

Techniques: Microarray, Isolation, Quantitative RT-PCR, Expressing, Saline

Journal: European Journal of Medical Research

Article Title: Differentially expressed long non-coding RNAs and mRNAs of cadmium exposure on learning disability of offspring rats

doi: 10.1186/s40001-024-01663-4

Figure Lengend Snippet: lncRNA and mRNA expression profiles. Heat map of differential expression ( A ) mRNAs and ( C ) LncRNAs. Each column represents a sample, while each row represents the degree of expression of a lncRNA or mRNA in a different sample. Red denotes high expression level, while blue denotes low expression.Volcano plots showing the up- and down-regulated B mRNAs and D lncRNA. The abscissa is -log10 (p value) and the ordinate is log2 (fold-change). Red denotes the up-regulated lncRNA/mRNAs, while blue denotes the down-regulated lncRNA/mRNAs, and black denotes no significant difference in gene expression. NC, non-treated control; lnc, long non-coding

Article Snippet: The CapitalBio Technology rat LncRNA array v1 is designed to have four identical arrays (8 × 60 K format) for each glass slide, each array containing about 30,254 rat mRNA probes and 22,088 LncRNA probes.

Techniques: Expressing, Quantitative Proteomics, Gene Expression, Control

Journal: European Journal of Medical Research

Article Title: Differentially expressed long non-coding RNAs and mRNAs of cadmium exposure on learning disability of offspring rats

doi: 10.1186/s40001-024-01663-4

Figure Lengend Snippet: LncRNA-mRNA co-expression network. Yellow circles represent lncRNAs, green circles represent mRNAs, the size of the circles represents the degree of genes in the network (number of neighbors), red lines represent positive correlations, and blue lines represent negative correlations

Article Snippet: The CapitalBio Technology rat LncRNA array v1 is designed to have four identical arrays (8 × 60 K format) for each glass slide, each array containing about 30,254 rat mRNA probes and 22,088 LncRNA probes.

Techniques: Expressing

Journal: Heliyon

Article Title: Microarray analysis of lncRNAs and mRNAs in spinal cord contusion rats with iPSC-derived A2B5 + oligodendrocyte precursor cells transplantation

doi: 10.1016/j.heliyon.2023.e22808

Figure Lengend Snippet: The mRNAs near the co-changed lncRNAs.

Article Snippet: The labelled cRNA was then hybridised onto a rat lncRNA Array v2.0 (8–60 K; ArrayStar, Rockville, MD, USA).

Techniques:

Journal: Heliyon

Article Title: Microarray analysis of lncRNAs and mRNAs in spinal cord contusion rats with iPSC-derived A2B5 + oligodendrocyte precursor cells transplantation

doi: 10.1016/j.heliyon.2023.e22808

Figure Lengend Snippet: The lncRNAs near the co-changed mRNAs.

Article Snippet: The labelled cRNA was then hybridised onto a rat lncRNA Array v2.0 (8–60 K; ArrayStar, Rockville, MD, USA).

Techniques:

Journal: Heliyon

Article Title: Microarray analysis of lncRNAs and mRNAs in spinal cord contusion rats with iPSC-derived A2B5 + oligodendrocyte precursor cells transplantation

doi: 10.1016/j.heliyon.2023.e22808

Figure Lengend Snippet: The intersected lncRNA and mRNA.

Article Snippet: The labelled cRNA was then hybridised onto a rat lncRNA Array v2.0 (8–60 K; ArrayStar, Rockville, MD, USA).

Techniques:

Journal: Heliyon

Article Title: Microarray analysis of lncRNAs and mRNAs in spinal cord contusion rats with iPSC-derived A2B5 + oligodendrocyte precursor cells transplantation

doi: 10.1016/j.heliyon.2023.e22808

Figure Lengend Snippet: The crucial lncRNA NR_037671 and mRNA Cntnap5a were validated by RT-qPCR. (A) Relative expression of lncRNA NR_037671 in Sham, SCC and iP-A2B5 + OPCs groups. (B) Relative expression of mRNA Cntnap5a in Sham, SCC and iP-A2B5 + OPCs groups. LncRNAs, long noncoding RNAs; iP-A2B5 + OPCs group, induced pluripotent stem cells-derived A2B5 + oligodendrocyte precursor cells; SCC, spinal cord contusion. Data were presented as mean ± SD, n = 6/group, * p < 0.05, ** p < 0.01.

Article Snippet: The labelled cRNA was then hybridised onto a rat lncRNA Array v2.0 (8–60 K; ArrayStar, Rockville, MD, USA).

Techniques: Quantitative RT-PCR, Expressing, Derivative Assay